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The Department of Transportation is primarily focused on ensuring safe and efficient transportation systems in the United States. Its main responsibilities include regulating transportation infrastructure, managing highways and public transit systems, and overseeing air and maritime transportation. These functions do not directly pertain to healthcare or public health initiatives.

In contrast, the other agencies listed have significant connections to healthcare. The Centers for Disease Control and Prevention (CDC) plays a critical role in public health surveillance, disease prevention, and the promotion of health safety measures. The Environmental Protection Agency (EPA) regulates environmental factors that can impact public health, addressing pollution and hazardous waste that may affect health outcomes. The Occupational Safety and Health Administration (OSHA) focuses on workplace health and safety regulations, ensuring that work environments are safe and do not pose health risks to employees.

Therefore, the Department of Transportation clearly does not align with healthcare-related activities, making it the option that is not associated with this field.